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Angiogenesis Protocols

Overview of attention for book
Cover of 'Angiogenesis Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Angiogenesis Protocols
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    Chapter 2 Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis
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    Chapter 3 Immunohistochemical Assessment of Leukocyte Involvement in Angiogenesis
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    Chapter 4 Isolation and Culture of Human Endothelial Cells from Micro- and Macro-vessels
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    Chapter 5 Isolation, Identification, and Culture of Human Lymphatic Endothelial Cells
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    Chapter 6 Isolation, Culture, and Characterization of Vascular Smooth Muscle Cells
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    Chapter 7 Isolation and Transfection of Primary Culture Bovine Retinal Pericytes
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    Chapter 8 In Vitro Assays for Endothelial Cell Functions Required for Angiogenesis: Proliferation, Motility, Tubular Differentiation, and Matrix Proteolysis
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    Chapter 9 Tube-Forming Assays
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    Chapter 10 Angiogenesis Protocols
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    Chapter 11 Spheroid-Based In Vitro Angiogenesis Model
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    Chapter 12 Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study of Angiogenesis
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    Chapter 13 Angiogenesis Protocols
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    Chapter 14 A Modified Aortic Ring Assay to Assess Angiogenic Potential In Vitro
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    Chapter 15 Quantitative Imaging-Based Examination of Pericytes Controlling Endothelial Growth Dynamics and Angiogenesis
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    Chapter 16 Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature
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    Chapter 17 Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy
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    Chapter 18 Angiogenesis Protocols
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    Chapter 19 Chorioallantoic Membrane Microtumor Model to Study the Mechanisms of Tumor Angiogenesis, Vascular Permeability, and Tumor Cell Intravasation
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    Chapter 20 Angiogenesis Protocols
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    Chapter 21 Angiogenesis Protocols
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    Chapter 22 Models of Oxygen Induced Retinopathy in Rodents
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    Chapter 23 The Sponge Implant Model of Angiogenesis
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    Chapter 24 Angiogenesis Protocols
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    Chapter 25 Angiogenesis Protocols
  27. Altmetric Badge
    Chapter 26 Use of the Hollow Fiber Assay to Evaluate Agents That Target the Tumor Neovasculature
  28. Altmetric Badge
    Chapter 27 Studying Vascular Angiogenesis and Senescence in Zebrafish Embryos
Attention for Chapter 4: Isolation and Culture of Human Endothelial Cells from Micro- and Macro-vessels
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Chapter title
Isolation and Culture of Human Endothelial Cells from Micro- and Macro-vessels
Chapter number 4
Book title
Angiogenesis Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3628-1_4
Pubmed ID
Book ISBNs
978-1-4939-3626-7, 978-1-4939-3628-1
Authors

Peter W. Hewett

Abstract

The endothelium from different vascular beds exhibits a high degree of phenotypic heterogeneity. Endothelial cells (EC) can be harvested easily from large vessels by mechanical removal or collagenase digestion. In particular, the human umbilical vein has been used due to its wide availability, and the study of ECs derived from it has undoubtedly greatly advanced our knowledge of vascular biology. However, the majority of the body's endothelium (>95 %) forms the microvasculature, and it is these cells providing the interface between the blood and tissues that play a critical role in the development of new blood vessels. This has led to the establishment of techniques for the isolation of microvascular ECs (MEC) from different tissues to provide more physiologically relevant in vitro models of angiogenesis and EC function.In this chapter the use of superparamagnetic beads (Dynabeads) coated with anti-PECAM-1 (CD31) antibodies (PECA-beads) to culture MECs from human adipose tissue is described along with the standard methods used to characterize them. Adipose tissue is an ideal source of MECs as it is composed mainly of adipocytes with a very rich microvasculature and is easy to disaggregate. Furthermore, it can be obtained in large quantities during plastic surgery procedures. Adipose obtained at reduction mammoplasty or abdominoplasty is first dissected free of the connective tissue, minced finely, and subjected to collagenase type II digestion. The adipocytes are removed by centrifugation to obtain a microvessel rich pellet, which is further disaggregated with trypsin/EDTA solution. Following filtration to remove fragments of the connective tissue, the pellet is incubated with PECA-beads and microvessel fragments/ECs and washed and harvested using a magnet. In addition, the adaptation of this basic technique for the isolation of the human lung and stomach MECs is also described along with common methods for the preparation of large vessel endothelial cells.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 21%
Researcher 5 18%
Student > Master 4 14%
Student > Bachelor 2 7%
Professor 1 4%
Other 2 7%
Unknown 8 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 25%
Medicine and Dentistry 6 21%
Agricultural and Biological Sciences 3 11%
Immunology and Microbiology 2 7%
Environmental Science 1 4%
Other 1 4%
Unknown 8 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 December 2017.
All research outputs
#15,485,255
of 23,011,300 outputs
Outputs from Methods in molecular biology
#5,388
of 13,157 outputs
Outputs of similar age
#232,186
of 394,701 outputs
Outputs of similar age from Methods in molecular biology
#546
of 1,471 outputs
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So far Altmetric has tracked 13,157 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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