Chapter title |
Angiogenesis Protocols
|
---|---|
Chapter number | 21 |
Book title |
Angiogenesis Protocols
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3628-1_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3626-7, 978-1-4939-3628-1
|
Authors |
Nakao, Shintaro, Hafezi-Moghadam, Ali, Shintaro Nakao, Ali Hafezi-Moghadam |
Abstract |
The cornea is a transparent tissue that lacks blood and lymphatic vessels. In addition, the cornea is readily accessible, which makes it convenient for direct visualization of angiogenesis and lymphangiogenesis. The corneal micropocket assay is a commonly used quantitative technique, in which a growth factor containing pellet is micro-surgically implanted into the cornea of a rodent. Subsequently, the growth of the preexisting limbal vessels toward the growth factor is visualized by live microscopy or immunohistochemistry. Recently, there has been significant interest in the process of lymphangiogenesis and the factors that regulate it. To facilitate these studies, we introduce a novel technique for visualization of the immune response during growth factor induced angiogenesis and lymphangiogenesis in the cornea. |
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