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Mendeley readers
Chapter title |
Isolation and Transfection of Primary Culture Bovine Retinal Pericytes
|
---|---|
Chapter number | 7 |
Book title |
Angiogenesis Protocols
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3628-1_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3626-7, 978-1-4939-3628-1
|
Authors |
Vincent A. Primo, Joseph F. Arboleda-Velasquez |
Abstract |
This protocol describes an enzymatic approach for isolating homogeneous cultures of pericytes from retinas of bovine source. In summary, retinas are dissected, washed, digested, filtered, cultured in specific media to select for pericytes, and finally expanded for a low passage culture of about 14 million bovine retinal pericytes (BRP) within 4-6 weeks. This protocol also describes a liposomal-based technique for transfection of BRPs. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 33% |
Professor | 1 | 17% |
Student > Ph. D. Student | 1 | 17% |
Student > Doctoral Student | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 33% |
Agricultural and Biological Sciences | 2 | 33% |
Medicine and Dentistry | 2 | 33% |