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Angiogenesis Protocols

Overview of attention for book
Cover of 'Angiogenesis Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Angiogenesis Protocols
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    Chapter 2 Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis
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    Chapter 3 Immunohistochemical Assessment of Leukocyte Involvement in Angiogenesis
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    Chapter 4 Isolation and Culture of Human Endothelial Cells from Micro- and Macro-vessels
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    Chapter 5 Isolation, Identification, and Culture of Human Lymphatic Endothelial Cells
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    Chapter 6 Isolation, Culture, and Characterization of Vascular Smooth Muscle Cells
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    Chapter 7 Isolation and Transfection of Primary Culture Bovine Retinal Pericytes
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    Chapter 8 In Vitro Assays for Endothelial Cell Functions Required for Angiogenesis: Proliferation, Motility, Tubular Differentiation, and Matrix Proteolysis
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    Chapter 9 Tube-Forming Assays
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    Chapter 10 Angiogenesis Protocols
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    Chapter 11 Spheroid-Based In Vitro Angiogenesis Model
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    Chapter 12 Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study of Angiogenesis
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    Chapter 13 Angiogenesis Protocols
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    Chapter 14 A Modified Aortic Ring Assay to Assess Angiogenic Potential In Vitro
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    Chapter 15 Quantitative Imaging-Based Examination of Pericytes Controlling Endothelial Growth Dynamics and Angiogenesis
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    Chapter 16 Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature
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    Chapter 17 Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy
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    Chapter 18 Angiogenesis Protocols
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    Chapter 19 Chorioallantoic Membrane Microtumor Model to Study the Mechanisms of Tumor Angiogenesis, Vascular Permeability, and Tumor Cell Intravasation
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    Chapter 20 Angiogenesis Protocols
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    Chapter 21 Angiogenesis Protocols
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    Chapter 22 Models of Oxygen Induced Retinopathy in Rodents
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    Chapter 23 The Sponge Implant Model of Angiogenesis
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    Chapter 24 Angiogenesis Protocols
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    Chapter 25 Angiogenesis Protocols
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    Chapter 26 Use of the Hollow Fiber Assay to Evaluate Agents That Target the Tumor Neovasculature
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    Chapter 27 Studying Vascular Angiogenesis and Senescence in Zebrafish Embryos
Attention for Chapter 24: Angiogenesis Protocols
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Chapter title
Angiogenesis Protocols
Chapter number 24
Book title
Angiogenesis Protocols
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3628-1_24
Pubmed ID
Book ISBNs
978-1-4939-3626-7, 978-1-4939-3628-1
Authors

Benest, Andrew V, Bates, David O, Andrew V. Benest, David O. Bates

Abstract

Successful therapeutic angiogenesis requires an understanding of how the myriad interactions of growth factors released during angiogenesis combine to form a mature vascular bed. This requires a model in which multiple physiological and cell biological parameters can be identified. The adenoviral-mediated mesenteric angiogenesis assay as described here is ideal for that purpose. The clear, thin, and relatively avascular mesenteric panel can be used to measure increased vessel perfusion by intravital microscopy. In addition, high-powered microvessel analysis is carried out by immunostaining of features essential for the study of angiogenesis or lymphangiogenesis (including endothelium, pericyte, smooth muscle cell area, and proliferation), allowing functional data to be obtained in conjunction with high-power microvessel ultrastructural analysis. Therefore, the mesenteric angiogenesis model offers a robust system to analyze the morphological changes associated with angiogenesis, induced by different agents.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Lecturer > Senior Lecturer 1 20%
Professor 1 20%
Student > Ph. D. Student 1 20%
Student > Master 1 20%
Student > Postgraduate 1 20%
Other 0 0%
Readers by discipline Count As %
Medicine and Dentistry 2 40%
Biochemistry, Genetics and Molecular Biology 1 20%
Neuroscience 1 20%
Agricultural and Biological Sciences 1 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2016.
All research outputs
#15,373,286
of 22,870,727 outputs
Outputs from Methods in molecular biology
#5,350
of 13,129 outputs
Outputs of similar age
#230,955
of 393,666 outputs
Outputs of similar age from Methods in molecular biology
#545
of 1,470 outputs
Altmetric has tracked 22,870,727 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,129 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.