| Chapter title |
Deciphering the Molecular Mechanism of the Bacteriophage φ 29 DNA Packaging Motor
|
|---|---|
| Chapter number | 13 |
| Book title |
Optical Tweezers
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6421-5_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6419-2, 978-1-4939-6421-5
|
| Authors |
Shixin Liu, Sara Tafoya, Carlos Bustamante, Liu, Shixin, Tafoya, Sara, Bustamante, Carlos |
| Abstract |
The past decade has seen an explosion in the use of single-molecule approaches to study complex biological processes. One such approach-optical trapping-is particularly well suited for investigating molecular motors, a diverse group of macromolecular complexes that convert chemical energy into mechanical work, thus playing key roles in virtually every aspect of cellular life. Here we describe how to use high-resolution optical tweezers to investigate the mechanism of the bacteriophage φ29 DNA packaging motor, a ring-shaped ATPase responsible for genome packing during viral assembly. This system illustrates how to use single-molecule techniques to uncover novel, often unexpected, principles of motor operation. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Postgraduate | 2 | 67% |
| Student > Doctoral Student | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 67% |
| Agricultural and Biological Sciences | 1 | 33% |