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Optical Tweezers

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Cover of 'Optical Tweezers'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Introduction to Optical Tweezers
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    Chapter 2 Exact Theory of Optical Tweezers and Its Application to Absolute Calibration
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    Chapter 3 Beyond the Hookean Spring Model: Direct Measurement of Optical Forces Through Light Momentum Changes
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    Chapter 4 A Surface-Coupled Optical Trap with 1-bp Precision via Active Stabilization
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    Chapter 5 Implementation and Tuning of an Optical Tweezers Force-Clamp Feedback System
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    Chapter 6 Custom-Made Microspheres for Optical Tweezers
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    Chapter 7 Optical Torque Wrench Design and Calibration
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    Chapter 8 High-Resolution “Fleezers”: Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection
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    Chapter 9 Versatile Quadruple-Trap Optical Tweezers for Dual DNA Experiments
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    Chapter 10 Probing DNA–DNA Interactions with a Combination of Quadruple-Trap Optical Tweezers and Microfluidics
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    Chapter 11 Probing Single Helicase Dynamics on Long Nucleic Acids Through Fluorescence-Force Measurement
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    Chapter 12 Mechanically Watching the ClpXP Proteolytic Machinery
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    Chapter 13 Deciphering the Molecular Mechanism of the Bacteriophage φ 29 DNA Packaging Motor
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    Chapter 14 Single-Molecule Protein Folding Experiments Using High-Precision Optical Tweezers
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    Chapter 15 Observing Single RNA Polymerase Molecules Down to Base-Pair Resolution
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    Chapter 16 Optical Tweezers-Based Measurements of Forces and Dynamics at Microtubule Ends
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    Chapter 17 Simultaneous Manipulation and Super-Resolution Fluorescence Imaging of Individual Kinetochores Coupled to Microtubule Tips
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    Chapter 18 Measurement of Force-Dependent Release Rates of Cytoskeletal Motors
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    Chapter 19 Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers
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    Chapter 20 Quantifying Force and Viscoelasticity Inside Living Cells Using an Active–Passive Calibrated Optical Trap
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    Chapter 21 Measuring Molecular Forces Using Calibrated Optical Tweezers in Living Cells
Attention for Chapter 2: Exact Theory of Optical Tweezers and Its Application to Absolute Calibration
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Chapter title
Exact Theory of Optical Tweezers and Its Application to Absolute Calibration
Chapter number 2
Book title
Optical Tweezers
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6421-5_2
Pubmed ID
Book ISBNs
978-1-4939-6419-2, 978-1-4939-6421-5
Authors

Rafael S. Dutra, Nathan B. Viana, Paulo A. Maia Neto, H. Moysés Nussenzveig, Dutra, Rafael S., Viana, Nathan B., Neto, Paulo A. Maia, Nussenzveig, H. Moysés

Abstract

Optical tweezers have become a powerful tool for basic and applied research in cell biology. Here, we describe an experimentally verified theory for the trapping forces generated by optical tweezers based on first principles that allows absolute calibration. For pedagogical reasons, the steps that led to the development of the theory over the past 15 years are outlined. The results are applicable to a broad range of microsphere radii, from the Rayleigh regime to the ray optics one, for different polarizations and trapping heights, including all commonly employed parameter domains. Protocols for implementing absolute calibration are given, explaining how to measure all required experimental parameters, and including a link to an applet for stiffness calculations.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 20%
Student > Postgraduate 1 20%
Student > Master 1 20%
Unknown 2 40%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 20%
Physics and Astronomy 1 20%
Unknown 3 60%