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Optical Tweezers

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Cover of 'Optical Tweezers'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Introduction to Optical Tweezers
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    Chapter 2 Exact Theory of Optical Tweezers and Its Application to Absolute Calibration
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    Chapter 3 Beyond the Hookean Spring Model: Direct Measurement of Optical Forces Through Light Momentum Changes
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    Chapter 4 A Surface-Coupled Optical Trap with 1-bp Precision via Active Stabilization
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    Chapter 5 Implementation and Tuning of an Optical Tweezers Force-Clamp Feedback System
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    Chapter 6 Custom-Made Microspheres for Optical Tweezers
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    Chapter 7 Optical Torque Wrench Design and Calibration
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    Chapter 8 High-Resolution “Fleezers”: Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection
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    Chapter 9 Versatile Quadruple-Trap Optical Tweezers for Dual DNA Experiments
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    Chapter 10 Probing DNA–DNA Interactions with a Combination of Quadruple-Trap Optical Tweezers and Microfluidics
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    Chapter 11 Probing Single Helicase Dynamics on Long Nucleic Acids Through Fluorescence-Force Measurement
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    Chapter 12 Mechanically Watching the ClpXP Proteolytic Machinery
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    Chapter 13 Deciphering the Molecular Mechanism of the Bacteriophage φ 29 DNA Packaging Motor
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    Chapter 14 Single-Molecule Protein Folding Experiments Using High-Precision Optical Tweezers
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    Chapter 15 Observing Single RNA Polymerase Molecules Down to Base-Pair Resolution
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    Chapter 16 Optical Tweezers-Based Measurements of Forces and Dynamics at Microtubule Ends
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    Chapter 17 Simultaneous Manipulation and Super-Resolution Fluorescence Imaging of Individual Kinetochores Coupled to Microtubule Tips
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    Chapter 18 Measurement of Force-Dependent Release Rates of Cytoskeletal Motors
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    Chapter 19 Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers
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    Chapter 20 Quantifying Force and Viscoelasticity Inside Living Cells Using an Active–Passive Calibrated Optical Trap
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    Chapter 21 Measuring Molecular Forces Using Calibrated Optical Tweezers in Living Cells
Attention for Chapter 6: Custom-Made Microspheres for Optical Tweezers
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Chapter title
Custom-Made Microspheres for Optical Tweezers
Chapter number 6
Book title
Optical Tweezers
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6421-5_6
Pubmed ID
Book ISBNs
978-1-4939-6419-2, 978-1-4939-6421-5
Authors

Anita Jannasch, Mohammad K. Abdosamadi, Avin Ramaiya, Suman De, Valentina Ferro, Aaron Sonnberger, Erik Schäffer, Jannasch, Anita, Abdosamadi, Mohammad K., Ramaiya, Avin, De, Suman, Ferro, Valentina, Sonnberger, Aaron, Schäffer, Erik

Abstract

Due to their high position and force sensitivity and the ability to remotely apply forces and torques, optical tweezers are widely used in diverse fields, such as biology, material science, and physics. Often, small dielectric particles are trapped and used as probes, which for experimental convenience are mostly spherical and composed of silica or polystyrene. The optical properties of these materials together with the microsphere size determine the trapping efficiency, and the position and force resolution. However, using only a single, homogeneous, isotropic, and unstructured material limits the range of trapping properties and thereby the applications of optical tweezers. Here, we show how custom-made microspheres composed of coated high-refractive-index materials-titania and nanodiamonds-and birefringent, liquid crystals extend the range and combination of desired trapping properties. These custom-made microspheres either enable the generation of high forces, a high force or time resolution, or the applications of torques. Custom-made probes expand the range of possible experiments and approaches broadening the scope and applicability of optical tweezers.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 33%
Researcher 4 27%
Student > Master 2 13%
Professor 1 7%
Student > Bachelor 1 7%
Other 1 7%
Unknown 1 7%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 27%
Physics and Astronomy 3 20%
Chemistry 3 20%
Medicine and Dentistry 1 7%
Agricultural and Biological Sciences 1 7%
Other 2 13%
Unknown 1 7%