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Mendeley readers
| Chapter title |
Measuring Molecular Forces Using Calibrated Optical Tweezers in Living Cells
|
|---|---|
| Chapter number | 21 |
| Book title |
Optical Tweezers
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6421-5_21 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6419-2, 978-1-4939-6421-5
|
| Authors |
Adam G. Hendricks, Yale E. Goldman, Hendricks, Adam G., Goldman, Yale E. |
| Abstract |
Optical tweezers have been instrumental in uncovering the mechanisms motor proteins use to generate and react to force. While optical traps have primarily been applied to purified, in vitro systems, emerging methods enable measurements in living cells where the actively fluctuating, viscoelastic environment and varying refractive index complicate calibration of the instrument. Here, we describe techniques to calibrate optical traps in living cells using the forced response to sinusoidal oscillations and spontaneous fluctuations, and to measure the forces exerted by endogenous ensembles of kinesin and dynein motor proteins as they transport cargoes in the cell. |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 54 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 24% |
| Student > Master | 9 | 17% |
| Researcher | 5 | 9% |
| Professor | 5 | 9% |
| Student > Bachelor | 3 | 6% |
| Other | 5 | 9% |
| Unknown | 14 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Physics and Astronomy | 14 | 26% |
| Agricultural and Biological Sciences | 10 | 19% |
| Biochemistry, Genetics and Molecular Biology | 8 | 15% |
| Engineering | 3 | 6% |
| Energy | 1 | 2% |
| Other | 3 | 6% |
| Unknown | 15 | 28% |