Chapter title |
Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers
|
---|---|
Chapter number | 19 |
Book title |
Optical Tweezers
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6421-5_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6419-2, 978-1-4939-6421-5
|
Authors |
Michael J. Greenberg, Henry Shuman, E. Michael Ostap, Greenberg, Michael J., Shuman, Henry, Ostap, E. Michael |
Abstract |
The myosin superfamily of molecular motors utilizes energy from ATP hydrolysis to generate force and motility along actin filaments in a diverse array of cellular processes. These motors are structurally, kinetically, and mechanically tuned to their specific molecular roles in the cell. Optical trapping techniques have played a central role in elucidating the mechanisms by which myosins generate force and in exposing the remarkable diversity of myosin functions. Here, we present thorough methods for measuring and analyzing interactions between actin and non-processive myosins using optical trapping techniques. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 22 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 23% |
Student > Bachelor | 4 | 18% |
Professor | 2 | 9% |
Lecturer > Senior Lecturer | 1 | 5% |
Other | 1 | 5% |
Other | 1 | 5% |
Unknown | 8 | 36% |
Readers by discipline | Count | As % |
---|---|---|
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Agricultural and Biological Sciences | 4 | 18% |
Nursing and Health Professions | 2 | 9% |
Chemistry | 1 | 5% |
Engineering | 1 | 5% |
Other | 0 | 0% |
Unknown | 8 | 36% |