Chapter title |
A Surface-Coupled Optical Trap with 1-bp Precision via Active Stabilization
|
---|---|
Chapter number | 4 |
Book title |
Optical Tweezers
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6421-5_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6419-2, 978-1-4939-6421-5
|
Authors |
Stephen R. Okoniewski, Ashley R. Carter, Thomas T. Perkins, Okoniewski, Stephen R., Carter, Ashley R., Perkins, Thomas T. |
Abstract |
Optical traps can measure bead motions with Å-scale precision. However, using this level of precision to infer 1-bp motion of molecular motors along DNA is difficult, since a variety of noise sources degrade instrumental stability. In this chapter, we detail how to improve instrumental stability by (1) minimizing laser pointing, mode, polarization, and intensity noise using an acousto-optical-modulator mediated feedback loop and (2) minimizing sample motion relative to the optical trap using a three-axis piezo-electric-stage mediated feedback loop. These active techniques play a critical role in achieving a surface stability of 1 Å in 3D over tens of seconds and a 1-bp stability and precision in a surface-coupled optical trap over a broad bandwidth (Δf = 0.03-2 Hz) at low force (6 pN). These active stabilization techniques can also aid other biophysical assays that would benefit from improved laser stability and/or Å-scale sample stability, such as atomic force microscopy and super-resolution imaging. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 25% |
Unknown | 3 | 75% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 1 | 25% |
Unknown | 3 | 75% |