Chapter title |
Employment of Salmonella in Cancer Gene Therapy
|
---|---|
Chapter number | 8 |
Book title |
Bacterial Therapy of Cancer
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3515-4_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3513-0, 978-1-4939-3515-4
|
Authors |
Che-Hsin Lee |
Abstract |
One of the primary limitations of cancer gene therapy is lack of selectivity of the therapeutic gene to tumor cells. Current efforts are focused on discovering and developing tumor-targeting vectors that selectively target only cancer cells but spare normal cells to improve the therapeutic index. The use of preferentially tumor-targeting bacteria as vectors is one of the innovative approaches for the treatment of cancer. This is based on the observation that some obligate or facultative-anaerobic bacteria are capable of multiplying selectively in tumors and inhibiting their growth. In this study, we exploited attenuated Salmonella as a tumoricidal agent and a vector to deliver genes for tumor-targeted gene therapy. Attenuated Salmonella, carrying a eukaryotic expression plasmid encoding an anti-angiogenic gene, was used to evaluate its' ability for tumor targeting and gene delivery in murine tumor models. We also investigated the use of a polymer to modify or shield Salmonella from the pre-existing immune response in the host in order to improve gene delivery to the tumor. These results suggest that tumor-targeted gene therapy using Salmonella carrying a therapeutic gene, which exerts tumoricidal and anti-angiogenic activities, represents a promising strategy for the treatment of tumors. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 36% |
Student > Bachelor | 2 | 18% |
Student > Doctoral Student | 1 | 9% |
Student > Master | 1 | 9% |
Student > Postgraduate | 1 | 9% |
Other | 0 | 0% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 36% |
Agricultural and Biological Sciences | 2 | 18% |
Veterinary Science and Veterinary Medicine | 1 | 9% |
Economics, Econometrics and Finance | 1 | 9% |
Medicine and Dentistry | 1 | 9% |
Other | 0 | 0% |
Unknown | 2 | 18% |