Chapter title |
Microfluidic Device to Quantify the Behavior of Therapeutic Bacteria in Three-Dimensional Tumor Tissue.
|
---|---|
Chapter number | 4 |
Book title |
Bacterial Therapy of Cancer
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3515-4_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3513-0, 978-1-4939-3515-4
|
Authors |
Brackett, Emily L, Swofford, Charles A, Forbes, Neil S, Emily L. Brackett, Charles A. Swofford, Neil S. Forbes |
Abstract |
Microfluidic devices enable precise quantification of the interactions between anti-cancer bacteria and tumor tissue. Direct observation of bacterial movement and gene expression in tissue is difficult with either monolayers of cells or tumor-bearing mice. Quantification of these interactions is necessary to understand the inherent mechanisms of bacterial targeting and to develop modified organisms with enhanced therapeutic properties. Here we describe the procedures for designing, printing, and assembling microfluidic tumor-on-a-chip devices. We also describe the procedures for inserting three-dimensional tumor-cell masses, exposure to bacteria, and analyzing the resultant images. |
X Demographics
Geographical breakdown
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 31% |
Researcher | 2 | 13% |
Student > Doctoral Student | 1 | 6% |
Lecturer | 1 | 6% |
Student > Master | 1 | 6% |
Other | 1 | 6% |
Unknown | 5 | 31% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 4 | 25% |
Engineering | 2 | 13% |
Biochemistry, Genetics and Molecular Biology | 2 | 13% |
Chemical Engineering | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Other | 1 | 6% |
Unknown | 5 | 31% |