You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
Development of a Targeted Gene-Delivery System Using Escherichia coli
|
|---|---|
| Chapter number | 9 |
| Book title |
Bacterial Therapy of Cancer
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3515-4_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3513-0, 978-1-4939-3515-4
|
| Authors |
Chung-Jen Chiang, Chih-Hsiang Chang, Yun-Peng Chao, Ming-Ching Kao |
| Abstract |
A gene-delivery system based on microbes is useful for development of targeted gene therapy of non-phagocytic cancer cells. Here, the feasibility of the delivery system is illustrated by targeted delivery of a transgene (i.e., eukaryotic GFP) by Escherichia coli to HER2/neu-positive cancer cells. An E. coli strain was engineered with surface display of the anti-HER2/neu affibody. To release the gene cargo, a programmed lysis system based on phage ϕX174 gene E was introduced into the E. coli strain. As a result, 3 % of HER2/neu-positive cells that were infected with engineered E. coli were able to express the GFP. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 22% |
| Lecturer | 1 | 11% |
| Student > Master | 1 | 11% |
| Student > Ph. D. Student | 1 | 11% |
| Professor > Associate Professor | 1 | 11% |
| Other | 1 | 11% |
| Unknown | 2 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 33% |
| Agricultural and Biological Sciences | 2 | 22% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 11% |
| Unknown | 3 | 33% |