Chapter title |
Human Cells as Platform to Produce Gamma-Carboxylated Proteins
|
---|---|
Chapter number | 4 |
Book title |
Recombinant Glycoprotein Production
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7312-5_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7311-8, 978-1-4939-7312-5
|
Authors |
Aline de Sousa Bomfim, Marcela Cristina Corrêa de Freitas, Dimas Tadeu Covas, Elisa Maria de Sousa Russo |
Abstract |
The gamma-carboxylated proteins belong to a family of proteins that depend on vitamin K for normal biosynthesis. The major representative gamma-carboxylated proteins are the coagulation system proteins, for example, factor VII, factor IX, factor X, prothrombin, and proteins C, S, and Z. These molecules have harbored posttranslational modifications, such as glycosylation and gamma-carboxylation, and for this reason they need to be produced in mammalian cell lines. Human cells lines have emerged as the most promising alternative to the production of gamma-carboxylated proteins. In this chapter, the methods to generate human cells as a platform to produce gamma-carboxylated proteins, for example the coagulation factors VII and IX, are presented. From the cell line modification up to the vitamin K adaptation of the produced cells is described in the protocols presented in this chapter. |
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