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Mendeley readers
| Chapter title |
Purification Methods for Recombinant Factor VIII Expressed in Human Liver SK-Hep Cells
|
|---|---|
| Chapter number | 15 |
| Book title |
Recombinant Glycoprotein Production
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7312-5_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7311-8, 978-1-4939-7312-5
|
| Authors |
Vladimir Granovski, Mario Soares Abreu-Neto, Dimas Tadeu Covas |
| Abstract |
Coagulation factor VIII is one of the largest proteins attempted to be expressed in recombinant form. A very complex and labile protein which has a very short half-live and need a fast and efficient purification chain. Here, we describe a simple purification sequence using multimodal Capto MMC, affinity FVIII select and ion exchange SP-Fastflow chromatography steps without subjecting the target molecule to mechanical and temperature stress, separating impurities from rFVIII using net charge, hydrophobicity, and affinity of the molecules. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 1 | 33% |
| Researcher | 1 | 33% |
| Unknown | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 33% |
| Medicine and Dentistry | 1 | 33% |
| Unknown | 1 | 33% |