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Recombinant Glycoprotein Production

Overview of attention for book
Cover of 'Recombinant Glycoprotein Production'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Platforms for Recombinant Therapeutic Glycoprotein Production
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    Chapter 2 Uncovering Innovation Features and Emerging Technologies in Molecular Biology through Patent Analysis
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    Chapter 3 Production of Full-Length Antibody by Pichia pastoris
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    Chapter 4 Human Cells as Platform to Produce Gamma-Carboxylated Proteins
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    Chapter 5 Production of Recombinant Factor VIII in Human Cell Lines
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    Chapter 6 Strategies to Suspension Serum-Free Adaptation of Mammalian Cell Lines for Recombinant Glycoprotein Production
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    Chapter 7 Production of Recombinant Rabies Virus Glycoprotein by Insect Cells in a Single-Use Fixed-Bed Bioreactor
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    Chapter 8 Cell-Free Production of Protein Biologics Within 24 H
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    Chapter 9 Demonstration-Scale High-Cell-Density Fermentation of Pichia pastoris
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    Chapter 10 Large-Scale Transient Transfection of Suspension Mammalian Cells for VLP Production
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    Chapter 11 Bioreactor-Based Production of Glycoproteins in Plant Cell Suspension Cultures
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    Chapter 12 Fed-Batch CHO Cell Culture for Lab-Scale Antibody Production
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    Chapter 13 Strategies to Develop Therapeutic N- and O-Hyperglycosylated Proteins
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    Chapter 14 Expression of Glycosylated Proteins in Bacterial System and Purification by Affinity Chromatography
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    Chapter 15 Purification Methods for Recombinant Factor VIII Expressed in Human Liver SK-Hep Cells
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    Chapter 16 Purification Method for Recombinant hG-CSF by Affinity Chromatography
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    Chapter 17 Microplate-Based Method for High-Throughput Screening (HTS) of Chromatographic Conditions Studies for Recombinant Protein Purification
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    Chapter 18 Purification and Autoactivation Method for Recombinant Coagulation Factor VII
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    Chapter 19 Preparation of Immunoliposomes by Direct Coupling of Antibodies Based on a Thioether Bond
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    Chapter 20 Polyester-Based Nanoparticles for the Encapsulation of Monoclonal Antibodies
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    Chapter 21 Polyester-Based Nanoparticles for Delivery of Therapeutic Proteins
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    Chapter 22 Quantification of Coagulation Factor VIII by Selective Reaction Monitoring
Attention for Chapter 12: Fed-Batch CHO Cell Culture for Lab-Scale Antibody Production
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Chapter title
Fed-Batch CHO Cell Culture for Lab-Scale Antibody Production
Chapter number 12
Book title
Recombinant Glycoprotein Production
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7312-5_12
Pubmed ID
Book ISBNs
978-1-4939-7311-8, 978-1-4939-7312-5
Authors

Yuzhou Fan, Daniel Ley, Mikael Rørdam Andersen

Abstract

Fed-batch culture is the most commonly used upstream process in industry today for recombinant monoclonal antibody production using Chinese hamster ovary (CHO) cells. Developing and optimizing this process in the lab is crucial for establishing process knowledge, which enables rapid and predictable tech-transfer to manufacturing scale. In this chapter, we describe stepwise how to carry out fed-batch CHO cell culture for lab-scale antibody production.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 74 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 12 16%
Student > Master 12 16%
Student > Ph. D. Student 12 16%
Researcher 6 8%
Professor > Associate Professor 2 3%
Other 1 1%
Unknown 29 39%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 19 26%
Pharmacology, Toxicology and Pharmaceutical Science 7 9%
Agricultural and Biological Sciences 5 7%
Immunology and Microbiology 4 5%
Chemical Engineering 3 4%
Other 6 8%
Unknown 30 41%