| Chapter title |
Strategies to Suspension Serum-Free Adaptation of Mammalian Cell Lines for Recombinant Glycoprotein Production
|
|---|---|
| Chapter number | 6 |
| Book title |
Recombinant Glycoprotein Production
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7312-5_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7311-8, 978-1-4939-7312-5
|
| Authors |
Angelo Luis Caron, Rafael Tagé Biaggio, Kamilla Swiech |
| Abstract |
Serum-free suspension cultures are preferably required for recombinant protein production due to its readiness in upstream/downstream processing and scale-up, therefore increasing process productivity and competitiveness. This type of culture replaces traditional cell culturing as the presence of animal-derived components may introduce lot-a-lot variability and adventitious pathogens to the process. However, adapting cells to serum-free conditions is challenging, time-consuming, and cell line and medium dependent. In this chapter, we present different approaches that can be used to adapt mammalian cell lines from an anchorage-dependent serum supplemented culture to a suspension serum-free culture. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 70 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 12 | 17% |
| Researcher | 10 | 14% |
| Student > Master | 7 | 10% |
| Student > Doctoral Student | 2 | 3% |
| Student > Ph. D. Student | 2 | 3% |
| Other | 6 | 9% |
| Unknown | 31 | 44% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 19 | 27% |
| Agricultural and Biological Sciences | 5 | 7% |
| Chemical Engineering | 4 | 6% |
| Engineering | 2 | 3% |
| Arts and Humanities | 2 | 3% |
| Other | 4 | 6% |
| Unknown | 34 | 49% |