| Chapter title |
Strategies to Develop Therapeutic N- and O-Hyperglycosylated Proteins
|
|---|---|
| Chapter number | 13 |
| Book title |
Recombinant Glycoprotein Production
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7312-5_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7311-8, 978-1-4939-7312-5
|
| Authors |
Agustina Gugliotta, Natalia Ceaglio, Marina Etcheverrigaray, Ricardo Kratje, Marcos Oggero |
| Abstract |
Glycoengineering by N- and/or O-hyperglycosylation represents a procedure to introduce potential sites for adding N- and/or O-glycosyl structures to proteins with the aim of producing biotherapeutics with improved pharmacodynamic and pharmacokinetic properties. In this chapter, a detailed description of the steps routinely performed to generate new proteins having high content of N- and/or O-glycosyl moieties is carried out. The rational strategy involves the initial stage of designing N- and/or O-hyperglycosylated muteins to be expressed by mammalian cells and includes the upstream and downstream processing stages necessary to develop hyperglycosylated versions of the proteins of interest with the purpose of beginning the long road toward producing biobetters. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 17% |
| Student > Bachelor | 1 | 17% |
| Researcher | 1 | 17% |
| Student > Master | 1 | 17% |
| Unknown | 2 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemical Engineering | 1 | 17% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 17% |
| Agricultural and Biological Sciences | 1 | 17% |
| Unknown | 3 | 50% |