| Chapter title |
Microplate-Based Method for High-Throughput Screening (HTS) of Chromatographic Conditions Studies for Recombinant Protein Purification
|
|---|---|
| Chapter number | 17 |
| Book title |
Recombinant Glycoprotein Production
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7312-5_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7311-8, 978-1-4939-7312-5
|
| Authors |
Rimenys J. Carvalho, Thayana A. Cruz |
| Abstract |
High-throughput screening (HTS) systems have emerged as important tools to provide fast and low cost evaluation of several conditions at once since it requires small quantities of material and sample volumes. These characteristics are extremely valuable for experiments with large number of variables enabling the application of design of experiments (DoE) strategies or simple experimental planning approaches. Once, the capacity of HTS systems to mimic chromatographic purification steps was established, several studies were performed successfully including scale down purification. Here, we propose a method for studying different purification conditions that can be used for any recombinant protein, including complex and glycosylated proteins, using low binding filter microplates. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 18% |
| Researcher | 1 | 9% |
| Professor | 1 | 9% |
| Unknown | 7 | 64% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Unknown | 7 | 64% |