Chapter title |
Quantification of Coagulation Factor VIII by Selective Reaction Monitoring
|
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Chapter number | 22 |
Book title |
Recombinant Glycoprotein Production
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7312-5_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7311-8, 978-1-4939-7312-5
|
Authors |
Edson Galdino do Nascimento Filho, Guilherme Pauperio Lanfredi, Mário Soares Abreu-Neto, Dimas Tadeu Covas, Vitor Marcel Faça |
Abstract |
Coagulation factor VIII (FVIII) is an important glycoprotein involved in the extrinsic coagulation cascade. Mutations in FVIII gene results in hemophilia A, a recessive coagulation disorder that is clinically managed by administration of purified FVIII from blood donors or recombinant FVIII. Because of its fundamental therapeutic application, biotechnological production of FVIII requires rigid quality control and monitoring in patients and clinical trials. Here, we describe a protocol for a mass spectrometry based approach termed selective reaction monitoring (SRM) as an important alternative tool for accurate and sensitive quantitation of purified or recombinant FVIII. |
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