Chikungunya Virus
Springer New York
Chapter title |
Virus Isolation and Preparation of Sucrose-Banded Chikungunya Virus Samples for Transmission Electron Microscopy
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Chapter number | 14 |
Book title |
Chikungunya Virus
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Published in |
Methods in molecular biology, May 2016
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DOI | 10.1007/978-1-4939-3618-2_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3616-8, 978-1-4939-3618-2
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Authors |
Chang-Kweng Lim |
Editors |
Justin Jang Hann Chu, Swee Kim Ang |
Abstract |
Virus isolation and purification is an invaluable technique in virology to detect and characterize viruses. This chapter describes a large-scale Chikungunya virus (CHIKV) propagation and purification methods by using discontinuous sucrose gradient, and sample preparation for transmission electron microscopy. Sucrose-banding yields large quantities of high-titer (10(10) pfu/ml) CHIKV stocks. Such stocks are stable for years when stored at -70 °C. |
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