Chapter title |
Neutralization Assay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test
|
---|---|
Chapter number | 25 |
Book title |
Chikungunya Virus
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3618-2_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3616-8, 978-1-4939-3618-2
|
Authors |
Nor Azila Muhammad Azami, Meng Ling Moi, Tomohiko Takasaki |
Editors |
Justin Jang Hann Chu, Swee Kim Ang |
Abstract |
Neutralization assay is a technique that detects and quantifies neutralizing antibody in serum samples by calculating the percentage of reduction of virus activity, as the concentration of virus used is usually constant. Neutralizing antibody titer is conventionally determined by calculating the percentage reduction in total virus infectivity by counting and comparing number of plaques (localized area of infection due to cytopathic effect) with a standard amount of virus. Conventional neutralizing test uses plaque-reduction neutralization test (PRNT) to determine neutralizing antibody titers against Chikungunya virus (CHIKV). Here we describe the plaque reduction neutralization assay (PRNT) using Vero cell lines to obtain neutralizing antibody titers. |
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