Chapter title |
Expression and Purification of E2 Glycoprotein from Insect Cells (Sf9) for Use in Serology
|
---|---|
Chapter number | 5 |
Book title |
Chikungunya Virus
|
Published in |
Methods in molecular biology, May 2016
|
DOI | 10.1007/978-1-4939-3618-2_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3616-8, 978-1-4939-3618-2
|
Authors |
Chong Long Chua, I-Ching Sam, Yoke Fun Chan |
Editors |
Justin Jang Hann Chu, Swee Kim Ang |
Abstract |
Chikungunya virus (CHIKV) is a mosquito-borne arbovirus which poses a major threat to global public health. Definitive CHIKV diagnosis is crucial, especially in distinguishing the disease from dengue virus, which co-circulates in endemic areas and shares the same mosquito vectors. Laboratory diagnosis is mainly based on serological or molecular approaches. The E2 glycoprotein is a good candidate for serological diagnosis since it is the immunodominant antigen during the course of infection, and reacts with seropositive CHIKV sera. In this chapter, we describe the generation of stable clone Sf9 (Spodoptera frugiperda) cells expressing secreted, soluble, and native recombinant CHIKV E2 glycoprotein. We use direct plasmid expression in insect cells, rather than the traditional technique of generating recombinant baculovirus. This recombinant protein is useful for serological diagnosis of CHIKV infection. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 5% |
Unknown | 21 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 23% |
Other | 4 | 18% |
Professor > Associate Professor | 2 | 9% |
Student > Master | 2 | 9% |
Student > Doctoral Student | 1 | 5% |
Other | 2 | 9% |
Unknown | 6 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 32% |
Agricultural and Biological Sciences | 4 | 18% |
Immunology and Microbiology | 2 | 9% |
Arts and Humanities | 1 | 5% |
Economics, Econometrics and Finance | 1 | 5% |
Other | 1 | 5% |
Unknown | 6 | 27% |