Chapter title |
Propagation of Chikungunya Virus Using Mosquito Cells
|
---|---|
Chapter number | 8 |
Book title |
Chikungunya Virus
|
Published in |
Methods in molecular biology, May 2016
|
DOI | 10.1007/978-1-4939-3618-2_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3616-8, 978-1-4939-3618-2
|
Authors |
Swee Kim Ang, Shirley Lam, Justin Jang Hann Chu |
Editors |
Justin Jang Hann Chu, Swee Kim Ang |
Abstract |
Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that transmits in between a mosquito host vector to a primate host and then back to the mosquito host vector to complete its life cycle. Hence, CHIKV must be able to replicate in both host cellular systems that are genetically and biochemically distinct. The ability to grow and propagate the virus in high titers in the laboratory is fundamentally crucial in order to understand virus replication in different host cellular systems and many other CHIKV research areas. Here, we describe a method on CHIKV propagation using C6/36, a mosquito cell line derived from Aedes albopictus in both serum-containing and serum-free media. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 4% |
Unknown | 24 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 20% |
Other | 4 | 16% |
Student > Ph. D. Student | 3 | 12% |
Student > Bachelor | 2 | 8% |
Student > Doctoral Student | 2 | 8% |
Other | 5 | 20% |
Unknown | 4 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 20% |
Agricultural and Biological Sciences | 5 | 20% |
Immunology and Microbiology | 3 | 12% |
Medicine and Dentistry | 2 | 8% |
Arts and Humanities | 1 | 4% |
Other | 2 | 8% |
Unknown | 7 | 28% |