Chapter title |
Detection and Quantification of Chikungunya Virus by Real-Time RT-PCR Assay
|
---|---|
Chapter number | 10 |
Book title |
Chikungunya Virus
|
Published in |
Methods in molecular biology, May 2016
|
DOI | 10.1007/978-1-4939-3618-2_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3616-8, 978-1-4939-3618-2
|
Authors |
Seok Mui Wang, Ummul Haninah Ali, Shamala Devi Sekaran, Ravindran Thayan |
Editors |
Justin Jang Hann Chu, Swee Kim Ang |
Abstract |
Real-time PCR assay has many advantages over conventional PCR methods, including rapidity, quantitative measurement, low risk of contamination, high sensitivity, high specificity, and ease of standardization (Mackay et al., Nucleic Acids Res 30:1292-1305, 2002). The real-time PCR system relies upon the measurement of a fluorescent reporter during PCR, in which the amount of emitted fluorescence is directly proportional to the amount of the PCR product in a reaction (Gibsons et al., Genome Res 6:995-1001, 1996). Here, we describe the use of SYBR Green I-based and TaqMan(®) real-time reverse transcription polymerase chain reaction (RT-PCR) for the detection and quantification of Chikungunya virus (CHIKV). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 3% |
Unknown | 38 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 8 | 21% |
Student > Bachelor | 5 | 13% |
Student > Ph. D. Student | 5 | 13% |
Other | 4 | 10% |
Student > Master | 3 | 8% |
Other | 7 | 18% |
Unknown | 7 | 18% |
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Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 4 | 10% |
Unknown | 10 | 26% |