| Chapter title |
Using Bicistronic Baculovirus Expression Vector System to Screen the Compounds That Interfere with the Infection of Chikungunya Virus
|
|---|---|
| Chapter number | 24 |
| Book title |
Chikungunya Virus
|
| Published in |
Methods in molecular biology, May 2016
|
| DOI | 10.1007/978-1-4939-3618-2_24 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3616-8, 978-1-4939-3618-2
|
| Authors |
Szu-Cheng Kuo, Chao-Yi Teng, Yi-Jung Ho, Ying-Ju Chen, Tzong-Yuan Wu |
| Editors |
Justin Jang Hann Chu, Swee Kim Ang |
| Abstract |
Chikungunya virus (CHIKV) is the etiologic agent of Chikungunya fever and has emerged in many countries over the past decade. There are no effective drugs for controlling the disease. A bicistronic baculovirus expression system was utilized to co-express CHIKV structural proteins C (capsid), E2 and E1 and the enhanced green fluorescence protein (EGFP) in Spodoptera frugiperda insect cells (Sf21). The EGFP-positive Sf21 cells fused with each other and with uninfected cells to form a syncytium is mediated by the CHIKV E1 allowing it to identify chemicals that can prevent syncytium formation. The compounds characterized by this method could be anti-CHIKV drugs. |
Mendeley readers
Geographical breakdown
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|---|---|---|
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| Unknown | 14 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 27% |
| Other | 3 | 20% |
| Student > Postgraduate | 3 | 20% |
| Student > Doctoral Student | 2 | 13% |
| Professor > Associate Professor | 2 | 13% |
| Other | 1 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Economics, Econometrics and Finance | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 2 | 13% |