Chapter title |
Chikungunya Virus
|
---|---|
Chapter number | 27 |
Book title |
Chikungunya Virus
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3618-2_27 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3616-8, 978-1-4939-3618-2
|
Authors |
Metz, Stefan W, Pijlman, Gorben P, Stefan W. Metz, Gorben P. Pijlman |
Editors |
Justin Jang Hann Chu, Swee Kim Ang |
Abstract |
Chikungunya virus is a reemerging human pathogen that causes debilitating arthritic disease in humans. Like dengue and Zika virus, CHIKV is transmitted by Aedes mosquitoes in an epidemic urban cycle, and is now rapidly spreading through the Americas since its introduction in the Caribbean in late 2013. There are no licensed vaccines or antiviral drugs available, and only a few vaccine candidates have passed Phase I human clinical trials. Using recombinant baculovirus expression technology, we have generated CHIKV glycoprotein subunit and virus-like particle (VLP) vaccines that are amenable to large scale production in insect cells. These vaccines, in particular the VLPs, have shown high immunogenicity and protection against CHIKV infection in different animal models of CHIKV-induced disease. Here, we describe the production, purification, and characterization of these potent CHIKV vaccine candidates. |
X Demographics
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United States | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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United States | 1 | 1% |
Brazil | 1 | 1% |
Unknown | 74 | 96% |
Demographic breakdown
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Researcher | 20 | 26% |
Student > Bachelor | 10 | 13% |
Student > Master | 8 | 10% |
Student > Doctoral Student | 7 | 9% |
Student > Ph. D. Student | 5 | 6% |
Other | 11 | 14% |
Unknown | 16 | 21% |
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Medicine and Dentistry | 9 | 12% |
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Mathematics | 2 | 3% |
Other | 11 | 14% |
Unknown | 19 | 25% |