Chapter title |
Chikungunya Virus
|
---|---|
Chapter number | 11 |
Book title |
Chikungunya Virus
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3618-2_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3616-8, 978-1-4939-3618-2
|
Authors |
Wong, Hui Vern, Chan, Yoke Fun, Sam, I-Ching, Sulaiman, Wan Yusof Wan, Vythilingam, Indra, Hui Vern Wong, Yoke Fun Chan, I-Ching Sam, Wan Yusof Wan Sulaiman, Indra Vythilingam |
Editors |
Justin Jang Hann Chu, Swee Kim Ang |
Abstract |
In vivo infection of mosquitoes is an important method to study and characterize arthropod-borne viruses. Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that is transmitted primarily by Aedes mosquitoes. In this chapter, we describe a protocol for infection of CHIKV in two species of Aedes mosquitoes, Aedes aegypti and Aedes albopictus, together with the isolation of CHIKV in different parts of the infected mosquito such as midgut, legs, wings, salivary gland, head, and saliva. This allows the study of viral infection, replication and dissemination within the mosquito vector. |
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