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Drug Target miRNA

Overview of attention for book
Cover of 'Drug Target miRNA'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 miRNA Targeting Drugs: The Next Blockbusters?
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    Chapter 2 Functional Analysis of miRNAs Using the DIANA Tools Online Suite.
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    Chapter 3 Non-nucleotide Modification of Anti-miRNA Oligonucleotides.
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    Chapter 4 Quantification of Oligonucleotide Association with miRNA-Argonaute Complexes In Vitro.
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    Chapter 5 Determination of Anti-miR Association with miRNA/Argonaute Complexes In Vivo.
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    Chapter 6 Competitive Argonaute-Based RNA Immunoprecipitation for Investigation of Transcriptomic Response to Anti-miR.
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    Chapter 7 Assessing Anti-miR Pharmacology with miRNA Polysome Shift Assay.
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    Chapter 8 Evaluating Synergistic Effects of miR-34a Mimics in Combination with Other Therapeutic Agents in Cultured Non-Small Cell Lung Cancer Cells.
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    Chapter 9 Assessing the Off-Target Effects of miRNA Inhibitors on Innate Immune Toll-Like Receptors.
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    Chapter 10 Design of Multimodal Small Molecules Targeting miRNAs Biogenesis: Synthesis and In Vitro Evaluation.
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    Chapter 11 Machine Learning Approaches Toward Building Predictive Models for Small Molecule Modulators of miRNA and Its Utility in Virtual Screening of Molecular Databases.
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    Chapter 12 Identification of Small Molecule Modulators of MicroRNA by Library Screening.
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    Chapter 13 Rapid Generation of miRNA Inhibitor Leads by Bioinformatics and Efficient High-Throughput Screening Methods.
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    Chapter 14 Drug Target miRNA
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    Chapter 15 Small Molecules Targeting the miRNA-Binding Domain of Argonaute 2: From Computer-Aided Molecular Design to RNA Immunoprecipitation.
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    Chapter 16 Surface Plasmon Resonance: A Useful Strategy for the Identification of Small Molecule Argonaute 2 Protein Binders.
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    Chapter 17 Antagonists of the miRNA-Argonaute 2 Protein Complex: Anti-miR-AGOs.
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    Chapter 18 Elucidating Mechanisms of Molecular Recognition Between Human Argonaute and miRNA Using Computational Approaches.
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    Chapter 19 Kinetic Analysis of Target RNA Binding and Slicing by Human Argonaute 2 Protein.
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    Chapter 20 Site-Specific Fluorescent Labeling of Argonaute for FRET-Based Bio-Assays.
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    Chapter 21 Single-Molecule Fluorescence Energy Transfer Assays for the Characterization of Reaction Pathways of miRNA-Argonaute Complex.
Attention for Chapter 20: Site-Specific Fluorescent Labeling of Argonaute for FRET-Based Bio-Assays.
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Chapter title
Site-Specific Fluorescent Labeling of Argonaute for FRET-Based Bio-Assays.
Chapter number 20
Book title
Drug Target miRNA
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6563-2_20
Pubmed ID
27924490
Book ISBNs
978-1-4939-6561-8, 978-1-4939-6563-2
Authors

Sarah Willkomm, Adrian Zander, Dina Grohmann

Editors

Marco F. Schmidt

Abstract

Deciphering the molecular mechanisms of eukaryotic Argonaute proteins is crucial for the understanding of RNA interference (RNAi), a posttranscriptional gene silencing process. Fluorescence-based single-molecule studies like single-molecule Förster resonance energy transfer (FRET) between a donor and acceptor dye represent a versatile tool to gain a mechanistic understanding of the structural dynamics of a biomolecular complex. Until today it was not possible to site-specifically introduce fluorophores into eukaryotic Argonaute. Using an archaeal Argonaute variant from Methanocaldococcus jannaschii that closely resembles its eukaryotic counterpart, we site-specifically incorporated fluorescent probes into Argonaute. In this chapter, we first describe how to express archaeal Argonaute with the site-specifically engineered unnatural amino acid para-azido-L-phenylalanine (pAzF) and subsequently describe the coupling of a fluorophore exploiting the unique chemistry of the azide group of pAzF. In the second part of the chapter, we present a methodological approach that probes complex formation between acceptor-labeled archaeal Argonaute and guide and target nucleic acids equipped with a donor fluorophore which ultimately allows single-molecule FRET measurements. Furthermore we describe binding and cleavage assays that report on the functionality of Argonaute-nucleic acid complexes.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 33 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 27%
Student > Master 7 21%
Researcher 5 15%
Other 2 6%
Student > Bachelor 2 6%
Other 3 9%
Unknown 5 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 12 36%
Agricultural and Biological Sciences 7 21%
Pharmacology, Toxicology and Pharmaceutical Science 3 9%
Chemistry 2 6%
Computer Science 1 3%
Other 3 9%
Unknown 5 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 January 2018.
All research outputs
#20,879,072
of 23,498,099 outputs
Outputs from Methods in molecular biology
#10,161
of 13,368 outputs
Outputs of similar age
#358,560
of 423,680 outputs
Outputs of similar age from Methods in molecular biology
#843
of 1,074 outputs
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So far Altmetric has tracked 13,368 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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