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Drug Target miRNA

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Cover of 'Drug Target miRNA'

Table of Contents

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    Book Overview
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    Chapter 1 miRNA Targeting Drugs: The Next Blockbusters?
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    Chapter 2 Functional Analysis of miRNAs Using the DIANA Tools Online Suite.
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    Chapter 3 Non-nucleotide Modification of Anti-miRNA Oligonucleotides.
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    Chapter 4 Quantification of Oligonucleotide Association with miRNA-Argonaute Complexes In Vitro.
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    Chapter 5 Determination of Anti-miR Association with miRNA/Argonaute Complexes In Vivo.
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    Chapter 6 Competitive Argonaute-Based RNA Immunoprecipitation for Investigation of Transcriptomic Response to Anti-miR.
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    Chapter 7 Assessing Anti-miR Pharmacology with miRNA Polysome Shift Assay.
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    Chapter 8 Evaluating Synergistic Effects of miR-34a Mimics in Combination with Other Therapeutic Agents in Cultured Non-Small Cell Lung Cancer Cells.
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    Chapter 9 Assessing the Off-Target Effects of miRNA Inhibitors on Innate Immune Toll-Like Receptors.
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    Chapter 10 Design of Multimodal Small Molecules Targeting miRNAs Biogenesis: Synthesis and In Vitro Evaluation.
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    Chapter 11 Machine Learning Approaches Toward Building Predictive Models for Small Molecule Modulators of miRNA and Its Utility in Virtual Screening of Molecular Databases.
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    Chapter 12 Identification of Small Molecule Modulators of MicroRNA by Library Screening.
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    Chapter 13 Rapid Generation of miRNA Inhibitor Leads by Bioinformatics and Efficient High-Throughput Screening Methods.
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    Chapter 14 Drug Target miRNA
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    Chapter 15 Small Molecules Targeting the miRNA-Binding Domain of Argonaute 2: From Computer-Aided Molecular Design to RNA Immunoprecipitation.
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    Chapter 16 Surface Plasmon Resonance: A Useful Strategy for the Identification of Small Molecule Argonaute 2 Protein Binders.
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    Chapter 17 Antagonists of the miRNA-Argonaute 2 Protein Complex: Anti-miR-AGOs.
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    Chapter 18 Elucidating Mechanisms of Molecular Recognition Between Human Argonaute and miRNA Using Computational Approaches.
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    Chapter 19 Kinetic Analysis of Target RNA Binding and Slicing by Human Argonaute 2 Protein.
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    Chapter 20 Site-Specific Fluorescent Labeling of Argonaute for FRET-Based Bio-Assays.
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    Chapter 21 Single-Molecule Fluorescence Energy Transfer Assays for the Characterization of Reaction Pathways of miRNA-Argonaute Complex.
Attention for Chapter 19: Kinetic Analysis of Target RNA Binding and Slicing by Human Argonaute 2 Protein.
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Chapter title
Kinetic Analysis of Target RNA Binding and Slicing by Human Argonaute 2 Protein.
Chapter number 19
Book title
Drug Target miRNA
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6563-2_19
Pubmed ID
27924489
Book ISBNs
978-1-4939-6561-8, 978-1-4939-6563-2
Authors

Sarah Willkomm, Tobias Restle

Editors

Marco F. Schmidt

Abstract

Analyzing the mechanisms of Argonaute-mediated gene silencing is essential to the understanding of RNA interference (RNAi). RNAi is a process to regulate gene expression on a posttranscriptional level. Directed by single-stranded small RNA guides, Argonaute 2 binds complementary target RNAs, and if the guide displays full complementarity to the targeted sequence, Argonaute 2 slices the bound target RNA. This on the one hand is an important mechanism to regulate gene expression in the cell and on the other hand represents a powerful tool to interfere with harmful gene expression levels. Here, we present techniques to kinetically characterize recombinant Argonaute 2-mediated guide and target binding as well as target RNA slicing. We focus on fluorescence-based steady-state and in particular pre-steady-state techniques to unravel mechanistic details. Furthermore, we describe a cleavage assay to analyze Argonaute 2-mediated slicing using radioactively labeled target strands.

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Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 100%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 50%
Chemistry 1 50%

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