Chapter title |
Surface Plasmon Resonance: A Useful Strategy for the Identification of Small Molecule Argonaute 2 Protein Binders.
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Chapter number | 16 |
Book title |
Drug Target miRNA
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6563-2_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6561-8, 978-1-4939-6563-2
|
Authors |
Elena Poser, Ilaria Genovese, Silvia Masciarelli, Teresa Bellissimo, Francesco Fazi, Gianni Colotti |
Editors |
Marco F. Schmidt |
Abstract |
Surface plasmon resonance (SPR) is one of the most important techniques for the detection and the characterization of molecular interactions. SPR technology is a label-free approach for monitoring biomolecular interactions in real time. The binding of analytes to molecules immobilized on a thin metal film (ligand) determines a change in the refractive index and, therefore in the angle of extinction of light, is reflected when polarized light hits the film, monitored in real time as a change in the position of the dip in reflected intensity. Since SPR detects mass, the technique is label-free.Here, we describe the use of SPR techniques to study the interaction between Argonaute 2 and small molecular compounds selected by means of high-throughput docking screening. |
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Unknown | 5 | 36% |
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