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Mendeley readers
| Chapter title |
Competitive Argonaute-Based RNA Immunoprecipitation for Investigation of Transcriptomic Response to Anti-miR.
|
|---|---|
| Chapter number | 6 |
| Book title |
Drug Target miRNA
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6563-2_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6561-8, 978-1-4939-6563-2
|
| Authors |
John R. Androsavich |
| Editors |
Marco F. Schmidt |
| Abstract |
Identification and validation of microRNA (miRNA) target genes is essential for gaining a better understanding of the many different functions miRNAs have in healthy and diseased cells. From a practical standpoint, validated target genes are also useful for monitoring pharmacological activity of developmental therapeutics that modulate miRNAs, such as anti-miRNA oligonucleotides (anti-miR). Here, we describe a method that uses changes in Argonaute 2-RNA immunoprecipitation in response to competition by anti-miR, titrated ex vivo, as physical evidence for target validation. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 33% |
| Other | 2 | 33% |
| Librarian | 1 | 17% |
| Student > Doctoral Student | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 33% |
| Agricultural and Biological Sciences | 1 | 17% |
| Sports and Recreations | 1 | 17% |
| Medicine and Dentistry | 1 | 17% |
| Neuroscience | 1 | 17% |
| Other | 0 | 0% |