Chapter title |
Small Molecules Targeting the miRNA-Binding Domain of Argonaute 2: From Computer-Aided Molecular Design to RNA Immunoprecipitation.
|
---|---|
Chapter number | 15 |
Book title |
Drug Target miRNA
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6563-2_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6561-8, 978-1-4939-6563-2
|
Authors |
Teresa Bellissimo, Silvia Masciarelli, Elena Poser, Ilaria Genovese, Alberto Del Rio, Gianni Colotti, Francesco Fazi |
Editors |
Marco F. Schmidt |
Abstract |
The development of small-molecule-based target therapy design for human disease and cancer is object of growing attention. Recently, specific microRNA (miRNA) mimicking compounds able to bind the miRNA-binding domain of Argonaute 2 protein (AGO2) to inhibit miRNA loading and its functional activity were described. Computer-aided molecular design techniques and RNA immunoprecipitation represent suitable approaches to identify and experimentally determine if a compound is able to impair the loading of miRNAs on AGO2 protein. Here, we describe these two methodologies that we recently used to select a specific compound able to interfere with the AGO2 functional activity and able to improve the retinoic acid-dependent myeloid differentiation of leukemic cells. |
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