↓ Skip to main content

RNA Vaccines

Overview of attention for book
Cover of 'RNA Vaccines'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Introduction to RNA Vaccines.
  3. Altmetric Badge
    Chapter 2 Self-Replicating RNA.
  4. Altmetric Badge
    Chapter 3 Self-Replicating RNA Vaccine Delivery to Dendritic Cells.
  5. Altmetric Badge
    Chapter 4 Plant Expression of Trans-Encapsidated Viral Nanoparticle Vaccines with Animal RNA Replicons.
  6. Altmetric Badge
    Chapter 5 RNActive® Technology: Generation and Testing of Stable and Immunogenic mRNA Vaccines.
  7. Altmetric Badge
    Chapter 6 Nucleoside Modified mRNA Vaccines for Infectious Diseases.
  8. Altmetric Badge
    Chapter 7 Generation and Evaluation of Prophylactic mRNA Vaccines Against Allergy.
  9. Altmetric Badge
    Chapter 8 Measuring the Adjuvant Activity of RNA Vaccines.
  10. Altmetric Badge
    Chapter 9 Generation of Immunostimulating 130 nm Protamine-RNA nanoparticles.
  11. Altmetric Badge
    Chapter 10 Electroporation of mRNA as Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins.
  12. Altmetric Badge
    Chapter 11 Adjuvant-Enhanced mRNA Vaccines.
  13. Altmetric Badge
    Chapter 12 Enhanced Delivery of DNA or RNA Vaccines by Electroporation.
  14. Altmetric Badge
    Chapter 13 The European Regulatory Environment of RNA-Based Vaccines.
  15. Altmetric Badge
    Chapter 14 Discovery and Subtyping of Neo-Epitope Specific T-Cell Responses for Cancer Immunotherapy: Addressing the Mutanome.
  16. Altmetric Badge
    Chapter 15 Considerations for Producing mRNA Vaccines for Clinical Trials.
  17. Altmetric Badge
    Chapter 16 Nonclinical Safety Testing of RNA Vaccines.
  18. Altmetric Badge
    Chapter 17 Immunotherapy of Uveal Melanoma: Vaccination Against Cancer.
Attention for Chapter 9: Generation of Immunostimulating 130 nm Protamine-RNA nanoparticles.
Altmetric Badge

Citations

dimensions_citation
10 Dimensions

Readers on

mendeley
14 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Generation of Immunostimulating 130 nm Protamine-RNA nanoparticles.
Chapter number 9
Book title
RNA Vaccines
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6481-9_9
Pubmed ID
Book ISBNs
978-1-4939-6479-6, 978-1-4939-6481-9
Authors

Marina Tusup, Steve Pascolo

Editors

Thomas Kramps, Knut Elbers

Abstract

Nanoparticles of defined size can be easily obtained by simply mixing Protamine, a pharmaceutical drug that is used to neutralize heparin after surgery, and RNA in the form of oligonucleotides or messenger RNA. Depending on the concentrations of the two reagents and their salt contents, homogenous nanoparticles with a mean diameter of 50 to more than 1000 nm can spontaneously be generated. RNA is a danger signal because it is an agonist of for example TLR-3, -7, and -8; therefore, Protamine-RNA nanoparticles are immunostimulating. We and others have shown in vitro that nanoparticle size and interferon-alpha production by human peripheral blood mononuclear cells (PBMCs) are inversely correlated. Conversely, nanoparticle size and TNF-alpha production by PBMCs are positively correlated (Rettig et al., Blood 115:4533-4541, 2010). Particles of less than 450 nm are most frequently used for research and clinical applications because they are very stable, remain polydispersed and induce interferon-alpha proteins, which are a natural antiviral and anticancer protein family with 12 members in humans. Herein, we describe a method to generate 130 nm nanoparticles as well as some of their physical and biological characteristics.

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 29%
Student > Bachelor 1 7%
Student > Doctoral Student 1 7%
Professor > Associate Professor 1 7%
Student > Postgraduate 1 7%
Other 0 0%
Unknown 6 43%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 43%
Medicine and Dentistry 1 7%
Unknown 7 50%