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RNA Vaccines

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Cover of 'RNA Vaccines'

Table of Contents

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    Book Overview
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    Chapter 1 Introduction to RNA Vaccines.
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    Chapter 2 Self-Replicating RNA.
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    Chapter 3 Self-Replicating RNA Vaccine Delivery to Dendritic Cells.
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    Chapter 4 Plant Expression of Trans-Encapsidated Viral Nanoparticle Vaccines with Animal RNA Replicons.
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    Chapter 5 RNActive® Technology: Generation and Testing of Stable and Immunogenic mRNA Vaccines.
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    Chapter 6 Nucleoside Modified mRNA Vaccines for Infectious Diseases.
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    Chapter 7 Generation and Evaluation of Prophylactic mRNA Vaccines Against Allergy.
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    Chapter 8 Measuring the Adjuvant Activity of RNA Vaccines.
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    Chapter 9 Generation of Immunostimulating 130 nm Protamine-RNA nanoparticles.
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    Chapter 10 Electroporation of mRNA as Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins.
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    Chapter 11 Adjuvant-Enhanced mRNA Vaccines.
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    Chapter 12 Enhanced Delivery of DNA or RNA Vaccines by Electroporation.
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    Chapter 13 The European Regulatory Environment of RNA-Based Vaccines.
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    Chapter 14 Discovery and Subtyping of Neo-Epitope Specific T-Cell Responses for Cancer Immunotherapy: Addressing the Mutanome.
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    Chapter 15 Considerations for Producing mRNA Vaccines for Clinical Trials.
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    Chapter 16 Nonclinical Safety Testing of RNA Vaccines.
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    Chapter 17 Immunotherapy of Uveal Melanoma: Vaccination Against Cancer.
Attention for Chapter 4: Plant Expression of Trans-Encapsidated Viral Nanoparticle Vaccines with Animal RNA Replicons.
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Chapter title
Plant Expression of Trans-Encapsidated Viral Nanoparticle Vaccines with Animal RNA Replicons.
Chapter number 4
Book title
RNA Vaccines
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6481-9_4
Pubmed ID
Book ISBNs
978-1-4939-6479-6, 978-1-4939-6481-9
Authors

Yiyang Zhou, Alison A. McCormick, Christopher M. Kearney

Editors

Thomas Kramps, Knut Elbers

Abstract

In this protocol, we outline how to produce a live viral nanoparticle vaccine in a biosafety level 1 (BSL1) environment. An animal viral vector RNA encapsidated with tobacco mosaic virus (TMV) coat protein can be fully assembled in planta. Agrobacterium cultures containing each component are inoculated together into tobacco leaves and the self-assembled hybrid nanoparticle vaccine is harvested 4 days later and purified with a simple PEG precipitation. The viral RNA delivery vector is derived from the BSL1 insect virus, Flock House virus (FHV), and replicates in human and animal cells but does not spread systemically. A polyethylene glycol purification protocol is also provided to collect and purify these vaccines for immunological tests.

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Lecturer 1 14%
Student > Doctoral Student 1 14%
Student > Bachelor 1 14%
Student > Master 1 14%
Researcher 1 14%
Other 1 14%
Unknown 1 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 29%
Pharmacology, Toxicology and Pharmaceutical Science 1 14%
Biochemistry, Genetics and Molecular Biology 1 14%
Chemistry 1 14%
Unknown 2 29%