| Chapter title |
Plant Expression of Trans-Encapsidated Viral Nanoparticle Vaccines with Animal RNA Replicons.
|
|---|---|
| Chapter number | 4 |
| Book title |
RNA Vaccines
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6481-9_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6479-6, 978-1-4939-6481-9
|
| Authors |
Yiyang Zhou, Alison A. McCormick, Christopher M. Kearney |
| Editors |
Thomas Kramps, Knut Elbers |
| Abstract |
In this protocol, we outline how to produce a live viral nanoparticle vaccine in a biosafety level 1 (BSL1) environment. An animal viral vector RNA encapsidated with tobacco mosaic virus (TMV) coat protein can be fully assembled in planta. Agrobacterium cultures containing each component are inoculated together into tobacco leaves and the self-assembled hybrid nanoparticle vaccine is harvested 4 days later and purified with a simple PEG precipitation. The viral RNA delivery vector is derived from the BSL1 insect virus, Flock House virus (FHV), and replicates in human and animal cells but does not spread systemically. A polyethylene glycol purification protocol is also provided to collect and purify these vaccines for immunological tests. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 8 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Lecturer | 1 | 13% |
| Student > Doctoral Student | 1 | 13% |
| Student > Bachelor | 1 | 13% |
| Student > Master | 1 | 13% |
| Researcher | 1 | 13% |
| Other | 1 | 13% |
| Unknown | 2 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 25% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 13% |
| Biochemistry, Genetics and Molecular Biology | 1 | 13% |
| Chemistry | 1 | 13% |
| Unknown | 3 | 38% |