Generation and Evaluation of Prophylactic mRNA Vaccines Against Allergy.
Methods in molecular biology, January 2017
Richard Weiss, Sandra Scheiblhofer, Josef Thalhamer, Weiss, Richard, Scheiblhofer, Sandra, Thalhamer, Josef
Thomas Kramps, Knut Elbers
Due to the worldwide increase in allergies and a limited efficacy of therapeutic interventions, the need for prophylactic vaccination against allergies has been recognized. mRNA and DNA vaccines have demonstrated their high potential for preventing allergic sensitization by inducing an immunological bias that prevents TH2 sensitization. However, only mRNA vaccines fulfill the stringent safety requirements for vaccination of healthy children. In this chapter, we describe the generation of conventional as well as self-replicating mRNA vaccines and methods to test their prophylactic efficacy in animal models.
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