| Chapter title |
Improving the Accuracy of Fitted Atomic Models in Cryo-EM Density Maps of Protein Assemblies Using Evolutionary Information from Aligned Homologous Proteins.
|
|---|---|
| Chapter number | 10 |
| Book title |
Data Mining Techniques for the Life Sciences
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3572-7_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3570-3, 978-1-4939-3572-7
|
| Authors |
Ramachandran Rakesh, Narayanaswamy Srinivasan |
| Editors |
Oliviero Carugo, Frank Eisenhaber |
| Abstract |
Cryo-Electron Microscopy (cryo-EM) has become an important technique to obtain structural insights into large macromolecular assemblies. However the resolution of the density maps do not allow for its interpretation at atomic level. Hence they are combined with high resolution structures along with information from other experimental or bioinformatics techniques to obtain pseudo-atomic models. Here, we describe the use of evolutionary conservation of residues as obtained from protein structures and alignments of homologous proteins to detect errors in the fitting of atomic structures as well as improve accuracy of the protein-protein interfacial regions in the cryo-EM density maps. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| New Zealand | 1 | 2% |
| Unknown | 48 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 15 | 31% |
| Student > Ph. D. Student | 12 | 24% |
| Student > Doctoral Student | 3 | 6% |
| Student > Master | 3 | 6% |
| Student > Bachelor | 2 | 4% |
| Other | 5 | 10% |
| Unknown | 9 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Chemistry | 3 | 6% |
| Medicine and Dentistry | 2 | 4% |
| Unspecified | 1 | 2% |
| Other | 4 | 8% |
| Unknown | 9 | 18% |