Preface. T-Cell Development.
Springer New York
Chapter title |
T-Cell Development
|
---|---|
Chapter number | 9 |
Book title |
T-Cell Development
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2809-5_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2808-8, 978-1-4939-2809-5
|
Authors |
Flomerfelt, Francis A, Gress, Ronald E, Francis A. Flomerfelt, Ronald E. Gress, Flomerfelt, Francis A., Gress, Ronald E. |
Abstract |
Radiation chimeras are prepared by subjecting recipient mice to sublethal or lethal dose of irradiation and injecting them with hematopoietic stem cells (HSC) from untreated donor mice. HSC can be obtained from bone marrow or fetal liver. This technique is a powerful tool when coupled with gene targeting strategies to investigate function of HSCs, thymocyte development, and T cell function. This protocol describes how to produce bone marrow or fetal liver chimeras. |
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