You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
Studying T Cell Development in Thymic Slices
|
|---|---|
| Chapter number | 11 |
| Book title |
T-Cell Development
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-2809-5_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2808-8, 978-1-4939-2809-5
|
| Authors |
Jenny O. Ross, Heather J. Melichar, Joanna Halkias, Ellen A. Robey |
| Abstract |
Recently, tissue slices have been adapted to study both mouse and human T cell development. Thymic slices combine and complement the strengths of existing organotypic culture systems to study thymocyte differentiation. Specifically, the thymic slice system allows for high throughput experiments and the ability to introduce homogenous developmental intermediate populations into an environment with a well-established cortex and medulla. These qualities make thymic slices a highly versatile and technically accessible model to study thymocyte development. Here we describe methods to prepare, embed, and slice thymic lobes to study T cell development in situ. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 25% |
| Student > Ph. D. Student | 5 | 25% |
| Student > Doctoral Student | 2 | 10% |
| Other | 1 | 5% |
| Professor | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 4 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 8 | 40% |
| Agricultural and Biological Sciences | 5 | 25% |
| Biochemistry, Genetics and Molecular Biology | 2 | 10% |
| Engineering | 1 | 5% |
| Unknown | 4 | 20% |