Chapter title |
Assessment of T Cell Development by Flow Cytometry
|
---|---|
Chapter number | 4 |
Book title |
T-Cell Development
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2809-5_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2808-8, 978-1-4939-2809-5
|
Authors |
Jan Y. M. Lee, Paul E. Love |
Abstract |
T cell development is a complex multistep process that requires the coordinated activation of distinct signaling responses and the regulated progression of developing cells (thymocytes) through key stages of maturation. Although sophisticated techniques such as fetal thymus organ culture, in vitro thymocyte culture, and multi-parameter flow cytometric analysis are now widely employed to evaluate thymocyte maturation by experienced laboratories, defects in T cell development can usually be identified with more simplified screening methods. Here, we provide a basic protocol for assessment of T cell development that will enable laboratories with access to a four parameter flow cytometer to screen mouse strains, including those generated from embryonic stem cells with targeted gene mutations, for thymocyte maturation defects. |
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Demographic breakdown
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