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Mendeley readers
| Chapter title |
Reconstituted Thymus Organ Culture
|
|---|---|
| Chapter number | 13 |
| Book title |
T-Cell Development
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-2809-5_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2808-8, 978-1-4939-2809-5
|
| Authors |
Zimu Deng, Haifeng Liu, Jinxiu Rui, Xiaolong Liu |
| Abstract |
Reconstituted thymus organ culture is based on fetal thymus organ culture (FTOC). Purified thymocyte populations, from genetically modified mice or even from other species, are cultured in vitro with thymic lobes depleted of their endogenous thymocytes (by 2'-deoxyguanosine treatment) to form a new thymus. This potent and timesaving method is distinct from FTOC, which assesses development of unmodified thymic lobes, and reaggregate thymic organ culture, in which epithelial cells are separately purified before being aggregated with thymocytes. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 30% |
| Professor | 1 | 10% |
| Other | 1 | 10% |
| Student > Master | 1 | 10% |
| Researcher | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 3 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 3 | 30% |
| Biochemistry, Genetics and Molecular Biology | 2 | 20% |
| Agricultural and Biological Sciences | 2 | 20% |
| Engineering | 1 | 10% |
| Unknown | 2 | 20% |