Chapter title |
In Vitro Analysis of Thymocyte Signaling
|
---|---|
Chapter number | 15 |
Book title |
T-Cell Development
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2809-5_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2808-8, 978-1-4939-2809-5
|
Authors |
Cody A. Cunningham, Emma Teixeiro, Mark A. Daniels |
Abstract |
From the moment a developing thymocyte expresses a TCR, it is subjected to numerous interactions with self-peptide/MHC complexes that determine its ultimate fate. These include death by neglect, negative selection (apoptosis and lineage deviation), positive selection, and lineage commitment. The identification of signals that govern these unique cell fates requires the ability to assess the activity, level of expression, subcellular location, and the molecular associations of numerous proteins within the developing T cell. Thus, this chapter describes methods designed to analyze thymocyte signaling under various types of peptide-based stimulation in vitro. |
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