Chapter title |
Analysis of Cell Proliferation and Homeostasis Using EdU Labeling
|
---|---|
Chapter number | 18 |
Book title |
T-Cell Development
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2809-5_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2808-8, 978-1-4939-2809-5
|
Authors |
Francis A. Flomerfelt, Ronald E. Gress |
Abstract |
Determination of cellular proliferation and population turnover is an important tool for research on lymphoid cell function. Historically this has been done using radiolabeled nucleotides or nucleoside analogs, such as BrdU (5-bromo-2-deoxyuridine), that are incorporated into nascent DNA during S-phase. Recently, a new procedure was developed to label nascent DNA using EdU (5-Ethynyl-2-deoxyuridine). This new method overcomes limitations imposed by the procedure used to detect BrdU because EdU detection is based on an easily performed chemical reaction that does not require DNA denaturation, is quick and reproducible, and has a superior signal-to-noise ratio. This technique offers a wide range of opportunities to analyze cellular proliferation, population homeostasis, and cell marking procedures. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 81 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 14 | 17% |
Student > Ph. D. Student | 10 | 12% |
Student > Master | 5 | 6% |
Student > Doctoral Student | 4 | 5% |
Researcher | 4 | 5% |
Other | 4 | 5% |
Unknown | 40 | 49% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 20 | 25% |
Immunology and Microbiology | 5 | 6% |
Agricultural and Biological Sciences | 3 | 4% |
Neuroscience | 3 | 4% |
Chemical Engineering | 2 | 2% |
Other | 7 | 9% |
Unknown | 41 | 51% |