| Chapter title |
Biophysics of Infection
|
|---|---|
| Chapter number | 3 |
| Book title |
Biophysics of Infection
|
| Published in |
Advances in experimental medicine and biology, May 2016
|
| DOI | 10.1007/978-3-319-32189-9_3 |
| Pubmed ID | |
| Book ISBNs |
978-3-31-932187-5, 978-3-31-932189-9
|
| Authors |
Thornton, K L, Findlay, R C, Walrad, P B, Wilson, L G, K. L. Thornton, R. C. Findlay, P. B. Walrad, L. G. Wilson, Thornton, K. L., Findlay, R. C., Walrad, P. B., Wilson, L. G. |
| Editors |
Mark C. Leake |
| Abstract |
To understand much of the behaviour of microbial pathogens, it is necessary to image living cells, their interactions with each other and with host cells. Species such as Escherichia coli are difficult subjects to image: they are typically microscopic, colourless and transparent. Traditional cell visualisation techniques such as fluorescent tagging or phase-contrast microscopy give excellent information on cell behaviour in two dimensions, but no information about cells moving in three dimensions. We review the use of digital holographic microscopy for three-dimensional imaging at high speeds, and demonstrate its use for capturing the shape and swimming behaviour of three important model pathogens: E. coli, Plasmodium spp. and Leishmania spp. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 43 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 26% |
| Researcher | 6 | 14% |
| Student > Bachelor | 5 | 12% |
| Student > Master | 5 | 12% |
| Other | 4 | 9% |
| Other | 6 | 14% |
| Unknown | 6 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Physics and Astronomy | 7 | 16% |
| Agricultural and Biological Sciences | 5 | 12% |
| Biochemistry, Genetics and Molecular Biology | 5 | 12% |
| Engineering | 5 | 12% |
| Medicine and Dentistry | 4 | 9% |
| Other | 10 | 23% |
| Unknown | 7 | 16% |