| Chapter title |
Biophysics of Infection
|
|---|---|
| Chapter number | 4 |
| Book title |
Biophysics of Infection
|
| Published in |
Advances in experimental medicine and biology, May 2016
|
| DOI | 10.1007/978-3-319-32189-9_4 |
| Pubmed ID | |
| Book ISBNs |
978-3-31-932187-5, 978-3-31-932189-9
|
| Authors |
Alys K Jepson, Jana Schwarz-Linek, Lloyd Ryan, Maxim G Ryadnov, Wilson C K Poon, Alys K. Jepson, Maxim G. Ryadnov, Wilson C. K. Poon |
| Editors |
Mark C. Leake |
| Abstract |
We measured the minimum inhibitory concentration (MIC) of the antimicrobial peptide pexiganan acting on Escherichia coli , and found an intrinsic variability in such measurements. These results led to a detailed study of the effect of pexiganan on the growth curve of E. coli, using a plate reader and manual plating (i.e. time-kill curves). The measured growth curves, together with single-cell observations and peptide depletion assays, suggested that addition of a sub-MIC concentration of pexiganan to a population of this bacterium killed a fraction of the cells, reducing peptide activity during the process, while leaving the remaining cells unaffected. This pharmacodynamic hypothesis suggests a considerable inoculum effect, which we quantified. Our results cast doubt on the use of the MIC as 'a measure of the concentration needed for peptide action' and show how 'coarse-grained' studies at the population level give vital information for the correct planning and interpretation of MIC measurements. |
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|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
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|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
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|---|---|---|
| Unknown | 34 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 18% |
| Student > Bachelor | 4 | 12% |
| Professor | 4 | 12% |
| Student > Master | 3 | 9% |
| Unspecified | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 13 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Biochemistry, Genetics and Molecular Biology | 3 | 9% |
| Unspecified | 1 | 3% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Other | 4 | 12% |
| Unknown | 15 | 44% |