Chapter title |
Using Biophysics to Monitor the Essential Protonmotive Force in Bacteria.
|
---|---|
Chapter number | 6 |
Book title |
Biophysics of Infection
|
Published in |
Advances in experimental medicine and biology, May 2016
|
DOI | 10.1007/978-3-319-32189-9_6 |
Pubmed ID | |
Book ISBNs |
978-3-31-932187-5, 978-3-31-932189-9
|
Authors |
Mei-Ting Chen, Chien-Jung Lo |
Editors |
Mark C. Leake |
Abstract |
Protonmotive force is an essential biological energy format in all levels of cells. Protonmotive force comprises electrical and chemical potential difference across biological membrane. In bacteria, protonmotive force couples to metabolism and ATP production. Moreover, protonmotive force directly provides driving energy of bacterial flagellar motor that is critical for bacterial motility and infection. Due to the small size of bacterial cells, there were limited experimental tools to measure protonmotive force in bacteria. Recent developments of optical membrane potential and intracellular pH indicators provide valuable information on bacterial studies. These new biophysical techniques allow us to monitor the protonmotive force even in single bacterial cell level that shed the light of next generation single-cell physiological experiments towards the understanding of bacterial infection process. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 33% |
Student > Bachelor | 2 | 33% |
Student > Ph. D. Student | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Physics and Astronomy | 2 | 33% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Agricultural and Biological Sciences | 1 | 17% |
Nursing and Health Professions | 1 | 17% |
Unknown | 1 | 17% |