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Mendeley readers
Chapter title |
Quantification of a Selective Expansion of T Cell Receptor Vβ by Superantigen Using Real-Time PCR
|
---|---|
Chapter number | 15 |
Book title |
Superantigens
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Published in |
Methods in molecular biology, December 2015
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DOI | 10.1007/978-1-4939-3344-0_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3342-6, 978-1-4939-3344-0
|
Authors |
Joo Youn Park, Keun Seok Seo |
Editors |
Amanda J. Brosnahan |
Abstract |
Selective expansion of T cells bearing specific T cell receptor Vβ segments is a hallmark of superantigens. Analyzing Vβ specificity of superantigens is important for characterizing newly discovered superantigens and understanding differential T cell responses to each toxin. Here, we describe a real-time PCR method using SYBR green I and primers specific to Cβ and Vβ genes for an absolute quantification. The established method was applied to quantify a selective expansion of T cell receptor Vβ expansion by superantigens and generated accurate, reproducible, and comparable results. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 75% |
Unspecified | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 25% |
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Agricultural and Biological Sciences | 1 | 25% |
Immunology and Microbiology | 1 | 25% |