| Chapter title |
Organ Culture as a Model System for Studies on Enterotoxin Interactions with the Intestinal Epithelium
|
|---|---|
| Chapter number | 14 |
| Book title |
Superantigens
|
| Published in |
Methods in molecular biology, December 2015
|
| DOI | 10.1007/978-1-4939-3344-0_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3342-6, 978-1-4939-3344-0
|
| Authors |
Ulver Spangsberg Lorenzen, Gert H. Hansen, E. Michael Danielsen |
| Editors |
Amanda J. Brosnahan |
| Abstract |
Studies on bacterial enterotoxin-epithelium interactions require model systems capable of mimicking the events occurring at the molecular and cellular levels during intoxication. In this chapter, we describe organ culture as an often neglected alternative to whole-animal experiments or enterocyte-like cell lines. Like cell culture, organ culture is versatile and suitable for studying rapidly occurring events, such as enterotoxin binding and uptake. In addition, it is advantageous in offering an epithelium with more authentic permeability/barrier properties than any cell line, as well as a subepithelial lamina propria, harboring the immune cells of the gut mucosa. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 50% |
| Unknown | 2 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 50% |
| Unknown | 2 | 50% |