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Breast Cancer

Overview of attention for book
Cover of 'Breast Cancer'

Table of Contents

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    Book Overview
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    Chapter 1 Basic Histopathological Methods and Breast Lesion Types for Research.
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    Chapter 2 Clinical Applications for Immunohistochemistry of Breast Lesions
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    Chapter 3 Immunohistochemistry for Triple-Negative Breast Cancer.
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    Chapter 4 In Situ Hybridization of Breast Cancer Markers.
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    Chapter 5 Evaluation of Human Epidermal Growth Factor Receptor 2 (HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Specimens by Fluorescence In Situ Hybridization (FISH).
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    Chapter 6 Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR).
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    Chapter 7 Detection of miRNA in Cultured Cells or Xenograft Tissues of Breast Cancer.
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    Chapter 8 Pyrosequencing Analysis for Breast Cancer DNA Methylome.
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    Chapter 9 Vita-Assay™ Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cells (CTCs).
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    Chapter 10 Breast Cancer Stem Cell Isolation.
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    Chapter 11 Cellular Apoptosis Assay of Breast Cancer.
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    Chapter 12 Assessment of Matrix Metalloproteinases by Gelatin Zymography.
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    Chapter 13 Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate Assay
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    Chapter 14 Determination of Breast Cancer Cell Migratory Ability.
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    Chapter 15 A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration
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    Chapter 16 Three-Dimensional Assay for Studying Breast Cancer Cell Invasion.
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    Chapter 17 A Combined Phagocytosis and Fluorescent Substrate Degradation Assay to Simultaneously Assess Cell Migration and Substrate Degradation
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    Chapter 18 Analysis of Invadopodia Formation in Breast Cancer Cells.
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    Chapter 19 Patient-Derived Tumor Xenograft Models of Breast Cancer.
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    Chapter 20 Monitoring Phosphatidic Acid Signaling in Breast Cancer Cells Using Genetically Encoded Biosensors.
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    Chapter 21 3D In Vitro Model for Breast Cancer Research Using Magnetic Levitation and Bioprinting Method
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    Chapter 22 Methods for Analyzing Tumor Angiogenesis in the Chick Chorioallantoic Membrane Model
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    Chapter 23 Pharmacokinetics and Pharmacodynamics in Breast Cancer Animal Models.
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    Chapter 24 Intracellular Delivery of Fluorescently Labeled Polysaccharide Nanoparticles to Cultured Breast Cancer Cells.
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    Chapter 25 Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression.
Attention for Chapter 12: Assessment of Matrix Metalloproteinases by Gelatin Zymography.
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Chapter title
Assessment of Matrix Metalloproteinases by Gelatin Zymography.
Chapter number 12
Book title
Breast Cancer
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3444-7_12
Pubmed ID
Book ISBNs
978-1-4939-3442-3, 978-1-4939-3444-7
Authors

Cathcart, Jillian, Jillian Cathcart

Abstract

Matrix metalloproteinases are endopeptidases responsible for remodeling of the extracellular matrix and have been identified as critical contributors to breast cancer progression. Gelatin zymography is a valuable tool which allows the analysis of MMP expression. In this approach, enzymes are resolved electrophoretically on a sodium dodecyl sulfate-polyacrylamide gel copolymerized with the substrate for the MMP of interest. Post electrophoresis, the enzymes are refolded in order for proteolysis of the incorporated substrate to occur. This assay yields valuable information about MMP isoforms or changes in activation and can be used to analyze the role of MMPs in normal versus pathological conditions.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 25%
Student > Bachelor 2 17%
Student > Doctoral Student 1 8%
Professor 1 8%
Lecturer 1 8%
Other 0 0%
Unknown 4 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 17%
Engineering 2 17%
Nursing and Health Professions 1 8%
Medicine and Dentistry 1 8%
Agricultural and Biological Sciences 1 8%
Other 0 0%
Unknown 5 42%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 January 2016.
All research outputs
#20,303,950
of 22,842,950 outputs
Outputs from Methods in molecular biology
#9,917
of 13,127 outputs
Outputs of similar age
#330,613
of 393,571 outputs
Outputs of similar age from Methods in molecular biology
#1,053
of 1,470 outputs
Altmetric has tracked 22,842,950 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,127 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.