Li, Yiyi, Wang, Kai, Chen, Longhua, Zhu, Xiaoxia, Zhou, Jie, Yiyi Li, Kai Wang, Longhua Chen, Xiaoxia Zhu, Jie Zhou
Abstract
Real-time quantitative reverse transcription PCR technique has advanced greatly over the past 20 years. Messenger RNA (mRNA) levels in cells or tissues can be quantified by this approach. It is well known that changes in mRNA expression in disease, and correlation of mRNA expression profiles with clinical parameters, serve as clinically relevant biomarkers. Hence, accurate determination of the mRNA levels is critically important in describing the biological, pathological, and clinical roles of genes in health and disease. This chapter describes a real-time PCR approach to detect and quantify mRNA expression levels, which can be used for both laboratorial and clinical studies in breast cancer research.
Pharmacology, Toxicology and Pharmaceutical Science
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8%
Psychology
1
8%
Unknown
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46%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 January 2016.
All research outputs
#20,303,950
of 22,842,950 outputs
Outputs from Methods in molecular biology
#9,917
of 13,127 outputs
Outputs of similar age
#330,613
of 393,571 outputs
Outputs of similar age from Methods in molecular biology
#1,053
of 1,470 outputs
Altmetric has tracked 22,842,950 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,127 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
